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                银纳米粒子用于无溶剂检测小分子和质荷比校准的激光解吸/电离质谱

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                发表于 2019-1-22 14:16:37 | 显示全部楼层 |阅读模式
                Silver nanoparticles for solvent-free detection of small molecules and mass-to-charge calibration of laser desorption/ionization mass spectrometry
                银纳米粒子用于无溶剂检测小分子和质荷比校准的激光解吸/电离质谱
                Journal of Vacuum Science & Technology B 37, 012906 (2019);


                Vadym Prysiazhnyi1,a), Filip Dycka1, Jiri Kratochvil1, Vitezslav Stranak1, Petra Ksirova2, and Zdenek Hubicka1
                Hide Affiliations
                1University of South Bohemia Faculty of Science, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic
                2Institute of Physics ASCR, Na Slovance 2, 18221 Prague 8, Czech Republic
                a)Author to whom correspondence should be addressed: vprysiazhnyi@prf.jcu.cz

                ABSTRACT
                摘要

                This work aims at the utilization of nanostructured surfaces for advanced mass spectrometry [laser desorption/ionization mass spectrometry (LDI MS)].。The authors demonstrate that prepared nanostructures enable independent mass-to-charge calibration and also effectively substitute protonation agent for low-mass molecules instead of conventionally used matrices. Silver nanostructured surfaces were formed as homogeneous thin film, isolated nanoislands, and spherical nanoparticles. Besides the surface characterization, the paper focuses on the impact of LDI MS laser, irradiating the nanostructured surfaces, which results in the production of charged Ag clusters. Irradiated nanoparticle-based surfaces mostly provide single ionized species Ag+ while positive (Agn+, n ≤ 5) and negative ions (Agn−, n ≤ 7) were observed from the nanoislands film. It is shown that the ratio between particular ion line intensities can be tailored by the deposition time. The pattern of silver ions Agn (due to two natural isotopes) can be used for mass-to-charge calibration up to 1000 m/z. Additionally, the silver protonation improves the identification of small molecules. It is demonstrated on detection of sucrose (342.3 g/mol), fructose (180.2 g/mol), and creatinine (113.1 g/mol) molecules.

                        这项工作旨在利用纳米结构表面进行高【级质谱分析[激光解吸/电离质谱(LDI MS)]。 作者证明,制备的纳米结构能够实现独立的质荷比校准,并且有效地替代用于低质量分子的质子化试剂,而不用常规的材〖料。形成了的银纳米结※构表面为均匀薄膜、分离的纳米岛和球形纳米颗粒。除了表面表征外,本文还重点研究了LDI MS激光照射纳米结构表面的说话有点结舌影响,由照射产生卐了带电的Ag团簇。受辐照的基于纳米颗粒的表面主要提供单电离物质Ag+,而从纳米岛膜层上可观察◎到正离子(Agn+, n ≤ 5)和负离子(Agn−, n ≤ 7)。这一结︾果表明,特定的出租车司机把送到了宾馆门前离子线强度之间的比例可以通过々沉积时间来调整。银离子Agn的模式(由于鼻子都被撞得塌了下去两种天然同位素)可用于高达◤1000 m/z的质荷比校准。另外,银质子化改善了小分子的识别水平。通过检测对蔗糖(342.3 g/mol),果糖(180.2 g/mol)和肌酸酐(113.1 g/mol)分子的检测证明了※这一点。



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